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matte249 106 – Fd. Medlem
Postad: 23 maj 2018 15:53

Hjälp att tolka forskningsrapport -elektrofores-

Hej 

Det står såhär:

This gene was chosen because of its large size (186 kb) and the availability of hemophiliac patients with well-characterized partial deletions. The sizes of three deletions estimated by PFGE (55 kb, 60 kb and 133 to 145 kb) were within 10% of the sizes calculated from conventional restriction analysis. Therefore, concatemers of lambda DNA and intact chromosomal DNA of Saccharomyces cerevisiae provide a relatively accurate system (within 10%) for sizing mammalian DNA fragments that are 100 to 550 kb in size. However, analysis of the fourth deletion (9 to 12 kb) revealed that it is difficult to detect changes in the size of mammalian DNA fragments of 8% or less using Southern blotting and PFGE.

 

Betyder detta att noggrannheten är 90% eller är precisionen 90%?

Mvh

 

Källa: https://www.researchgate.net/post/Agarose_gel_percentage_to_see_band_of_8_kb_and_01_kb

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